Composite

Part:BBa_K3799010

Designed by: Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-10-01)


AIP-1 sensor of S.aureus under pBAD promoter

This part consist of the AIP-1 sensing and signal transduction system of S aureus(BBa_I746001) under pveg,constitutive promoter, of B.subtilis(BBa_K143012).

The AgrC-AgrA is a two component signal transduction system which senses the AIP-1 molecule secreted by S.aureus.AgrC encodes a transmembrane histidine kinase receptor which binds to exogenous AIP-1 secreted by other S.aureus which inturn phosphorylates intracellular AgrA which acts as a signalling molecule which inturn activates the P2 and P3 promoter involved in quorum sensing,

This part can be used to replicate the two component signal transduction system from S.aureus into other host such as B.subtilis and Ecoli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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